Traction force-activated therapeutics (TrAPs)

Reference number 8021

Sectors: Pharmaceuticals

Industries: Pharmaceuticals

Proposed use

TrAPs technology has significant potential via repurposing existing inhibitory aptamers for new addressable tissue repair markets including skin repair, orthobiologics/orthopaedics, angiogenesis, and neural injury. Immediate potential markets could include spinal and ankle fusion, maxillofacial reconstruction, angiogenesis, and wound repair. Moreover, synthetic synthesis strategy instead of protein engineering offers attractive manufacturing opportunities.

Problem addressed

Growth factors (GFs) have long been investigated as potentially powerful therapeutics for promoting tissue repair. Currently, a handful of GFs are in various markets for promoting the repair of tissues including bone and skin. However, these treatments generally use inefficient methods of delivery. This leads to therapeutic doses of GFs that are orders-of-magnitude higher than physiologically relevant, leading to serious side-effects and prohibitively high manufacturing costs.

The TrAPs technology overcomes these limitations by creating an engineered platform for controllable, ‘cell-activated’ therapeutic delivery. It is the only technology available that harnesses cellular traction forces to activate therapeutics such as GF at physiologically relevant doses to locally promote tissue repair.

Technology overview

TrAPs are based on aptamers – single-stranded DNA/RNA molecules that fold into 3D secondary structures that bind and inhibit proteins with affinities and specificities rivalling antibodies, with their 3D folded structure being critical for their function. TrAPs are created by attaching a cell adhesion peptide to one end of the aptamer and the other end to a biomaterial substrate. This enables cells to directly bind the TrAPs and actively trigger the activation and release of bound proteins via traction forces that unfold the aptamer. This is a fundamentally different approach to GF delivery that has the potential to drastically reduce the GF dose necessary for therapeutic effect by increasing delivery efficacy, while also eliminating the serious side-effects associated with existing strategies.

Benefits

  • Chemically synthesised using established procedures for reduced manufacturing costs
  • Can potentially deliver any protein/peptide of therapeutic interest due to SELEX selection/enrichment process for aptamers
  • Predicted to reduce required therapeutic GF doses by several orders of magnitude over existing technologies due to controlled, on-demand cell activation and possible synergistic integrin signalling
  • Only technology to have demonstrated selective activation of GF by different cell types
  • Can be incorporated into/onto any biomaterial scaffold (e.g. collagen, fibrin, cellulose, silk, PEG, PLGA, PCL, PEEK, titanium)
  • Minimally-invasive

Intellectual property information

EP 17777320.7 – DRUG DELIVERY USING APTAMER CONSTRUCT

US 16/333982 – DRUG DELIVERY USING APTAMER CONSTRUCT

CN 110177574A – 使用适体构建体的药物递送

Link to published paper(s)

Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces

Anna Stejskalová, Nuria Oliva, Frances J. England, and Benjamin D. Almquist, Advanced Materials v31, 1806380 (2019). DOI: 10.1002/adma.201806380

Inventor

Dr Ben Almquist

Senior Lecturer
Faculty of Engineering, Department of Bioengineering

Visit personal site

Contact us about this technology



Contact

Diana Yin

Industry Partnerships and Commercialisation Executive, Engineering

Diana supports and leads on the commercialisation of MedTech, and HealthTech related technologies from Imperial’s Faculty of Engineering

Contact Diana

d.yin@imperial.ac.uk

Related technologies

A cytokine for wound healing and scar reduction

A cytokine for wound healing and scar reduction

A cytokine that promotes wound healing by accelerating re-epithelialization of the wound bed faster than FDA approved products. The treatment also has a potential to minimise scarring after injury. Find out more

A novel targeted drug delivery system

A novel targeted drug delivery system

Red blood cell-derived vesicles (RBCVs) for use in targeted delivery of thrombolytic drugs to blood clots. Find out more

A novel targeted drug delivery system

A novel targeted drug delivery system

A novel nanomedicine platform technology using biocompatible and biodegradable nanovesicles as carriers for a variety of clot-lysing thrombolytics Find out more

Sign up for updates

Sign up for monthly technology alerts via email, and find other ways to connect with us.

Loading...